Mechanism of Cell Growth Inhibition After the MDR1 Interfered by siRNA in Renal Cell Carcinoma

Objective To investigate the cell growth inhibition of the renal cell carcinoma following the small interference RNA fragment stabiling transfection,and explore the potential mechanism.Methods First,we transfered the RNA interference lentivirus particles into the target cells and made them stabling generating.Then we used the CCK - 8 kit to test the growth of the target cells and used the Real - time PCR to test the expression change of the P53 gene and the survivin gene.Results We find that the growth level of the interfered ACHN cell group was obviously lower than the other two control groups,and the cell vigor of the interfered group showed an obvious decrease.On the other hand,compared with the other two control groups,the mRNA expression level of the P53 gene and the survivin gene in the interfered group cells obviously depressed,but the NC group showed no much difference with the parent cells.Conclusions MDR1 gene silence can step down the cell proliferation of the renal cell carcinoma ACHN cells,and inhibit their growth.The expression of the mtP53 gene and survivin gene obviously deceased in the target cells when they were silenced.This demonstrated the MDR1 gene mediated inhibition would be implemented partially by the P53 gene and survivin gene.