Preparation of Porous Arrowroot Starch by Double Enzymatic Hydrolysis
Ganhui Huang
Abstract
Ganhui Huang
Abstract
Porous arrowroot starch was prepared by simultaneous hydrolysis with α-amylase and glucoamylase.The effects of enzyme dosage,glucoamylase-to-α-amylase ratio,pH,hydrolysis time and hydrolysis temperature on the oil-absorbing capacity of porous arrowroot starch were investigated.The optimal hydrolysis conditions for preparing porous arrowroot starch were determined by orthogonal array design method to be 3:1 glucoamylase-to-α-amylase ratio,0.6% total enzyme amount,pH 5.0,12 h hydrolysis time and 50 ℃ hydrolysis temperature.Under these conditions,the maximum oil-absorbing rate of 3:1 and excellent pore formation were achieved.
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Porous arrowroot starch was prepared by simultaneous hydrolysis with α-amylase and glucoamylase.The effects of enzyme dosage,glucoamylase-to-α-amylase ratio,pH,hydrolysis time and hydrolysis temperature on the oil-absorbing capacity of porous arrowroot starch were investigated.The optimal hydrolysis conditions for preparing porous arrowroot starch were determined by orthogonal array design method to be 3:1 glucoamylase-to-α-amylase ratio,0.6% total enzyme amount,pH 5.0,12 h hydrolysis time and 50 ℃ hydrolysis temperature.Under these conditions,the maximum oil-absorbing rate of 3:1 and excellent pore formation were achieved.
Key concepts: Hydrolysis, Starch, Chemistry, Enzymatic hydrolysis, Amylase, Chromatography, Alpha-amylase, Enzyme