Anti-inflammatory and antioxidant effect of ginsenoside Re on the lung injury induced by intestinal ischemia reperfusion in rats

Objective To study the anti-inflammatory and antioxidant effect of ginsenoside Re from Panax notoginseng(Burk.) F. H. Chen on the lung injury induced by intestinal ischemia/reperfusion( Ⅱ /R) in rats. Methods Thirty-two Sprague-Dawley male rats were randomly divided into 4 groups: a sham group, a Ⅱ /R model group, a Re-H group( Ⅱ /R + ginsenoside Re high-dose) and a Re-L group( Ⅱ /R + ginsenoside Re low-dose). A rat model of intestinal ischemia/reperfusion injury was seted up by 1 h of superior mesenteric artery occlusion followed by 2 h min of reperfusion. The serum samples were collected to measure the contents of TNF-α, IL-6, IL-10, MDA and SOD, while the lung tissues were harvested for morphological examination. Results Compared with the sham group, the lung tissue injury was significantly aggravated and the wet/dry ratio of lung tissue increased in M group(P 0.01), and the levels of TNF-α, IL-6, IL-10 and MDA in the serum were significantly increased in M group(P 0.05 or P 0.01), The activity of SOD was decreased markedly(P 0.01). When pretreated with ginsenoside Re, the changes of TNF-α, IL-6, IL-10, SOD and MDA levels were diminished markedly(P 0.05 or P 0.01), and the lung injury was markedly reduced. Conclusion Ginsenoside Re pretreatment can ameliorate the lung injury by improving the anti-oxidative and antiinflammatory abilities.