ScholarLens Back to search
2005•Fuzhou daxue xuebao. Ziran kexue banRequires access

Cloning and expression in E coli of murine tyrosinase

Liu S

Open publisher page 0 citations

Abstract

Murine tyrosinase cDNA was amplified from total RNA of the murine melanotic carcinoma by reverse transcriptase PCR(RT-PCR). The expression plasmid pET-TYR was constructed by genetically cloning the DNA fragment into expression vector pET-22b(+) which under the control of T7 promotor. The results of SDS-PAGE and protein sequencing showed that after induction by 0.8?mmol/L IPTG, recombinant murine tyrosinase was highly expressed in E coli Rosetta(DE3). The quantities of expression product was up to 25% of the total protein of the bacteria in insoluble bodies form.

About this research paper

What this paper is about

Murine tyrosinase cDNA was amplified from total RNA of the murine melanotic carcinoma by reverse transcriptase PCR(RT-PCR). The expression plasmid pET-TYR was constructed by genetically cloning the DNA fragment into expression vector pET-22b(+) which under the control of T7 promotor. The results of SDS-PAGE and protein sequencing showed that after induction by 0.8?mmol/L IPTG, recombinant murine tyrosinase was highly expressed in E coli Rosetta(DE3). The quantities of expression product was up to 25% of the total protein of the bacteria in insoluble bodies form.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Murine tyrosinase cDNA was amplified from total RNA of the murine melanotic carcinoma by reverse transcriptase PCR(RT-PCR). The expression plasmid pET-TYR was constructed by genetically cloning the DNA fragment into expression vector pET-22b(+) which under the control of T7 promotor. The results of SDS-PAGE and protein sequencing showed that after induction by 0.8?mmol/L IPTG, recombinant murine tyrosinase was highly expressed in E coli Rosetta(DE3). The quantities of expression product was up to 25% of the total protein of the bacteria in insoluble bodies form.

Key concepts: Molecular biology, Cloning (programming), Complementary DNA, Recombinant DNA, Expression vector, lac operon, Tyrosinase, Plasmid

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and expression in E coli of murine tyrosinase — Research Paper | ScholarLens