Multi-primers RT-PCR for the rapid detection and typing of dengue viruses

Objective: To establish the multi primer PCR assay for detection and typing of dengue viruses. Methods: Relative primers were synthesized according to conservative and type specific sequences of nucleotides of genomes of dengue viruses. RNAs of dengue viruses were amplified by means of RT PCR. Specificity and typing of amplification products were observed by electrophoresis. Results and conclusion: Specific bands of dengue viruses were obtained and the viruses could be typed by multi primers RT PCR. The results suggest that the assay can serve as a sensitive, specific, and rapid tool for laboratory and clinical use in endemic areas of dengue fever. [