Study on Effects of Acute Alcohol Administration on Serum Anti-oxidizing System of Mice and of Protection of Flavonoids Supplement Extracted from Ginkgo Biloba

Objective:To study the protective effects of flavonoids of ginkgo biloba on serum anti-oxidizing system induced by acute alcohol administration on mice. Methods :After being pretreated with flavonoids of ginkgo biloba (96mg/kgbw), the mice ingested alcohol (4.8g ethanol/kgbw) via i.g. and were decapitated after 0.5h, 1h, 2h, 4h, 6h, 9h, and 15h of last time of i.g respectively. Meanwhile, the normal control group and ethanol control group (4.8g ethanol/kgbw) were employed in the study. The concentration of glutathione (GSH), malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum were determined. Result: The experiment displayed that the content of GSH and the activities of GSH-Px and SOD decreased rapidly after 1h of treatment with alcohol and dropped to the lowest level at 4h of treatment. 6h after treatment, these indexes returned to the normal level rapidly. The level of MDA increased rapidly 1h after treatment and reached the climax at 4h. It returned to the normal concentration until 15h. On the whole, there were similar curves between flavonoids supplement group and alcohol control group on the indexes. However, to some extent, the supplement of flavonoid of ginkgo biloba could prohibit the rise of MDA level, while the decline of GSH-Px, SOD, GSH was much less than what were induced by acute alcohol intakes. Conclusion :Flavonoids of ginkgo biloba had some protective effects on the damage of serum anti-oxidizing system of mice induced by acute alcohol adminstration.