SRAP analysis of genetic diversity of four female populations in Torreya grandis

In order to provide a theoretical basis for breeding Torreya grandis, with 86 samples of T. grandis from four natural populations located in Zhuta and Taihuyuan of Zhejiang Province, Qiaoshan and Xiaorong of Anhui Province, based on optimal SRAP-PCR reaction system and amplification conditions, 26 pairs of primers were amplified. As a result, 404 loci were amplified, in which 330 polymorphic loci, accounting for 81.68% of the total, and average polymorphism information content value is 0.294 3. The results of POPGENE analysis show that Nei's genetic diversity index of populations is 0.294 9, Shannon's information index of populations is 0.454 5, and gene fl ow between populations is 4.090 9, which indicates that genetic diversity is overall high in this species. In addition, gene differentiation coeffi cient between populations is 0.108 9, so the majority of genetic variations occur within a population. Correlation between genetic distance and geographical distance among four populations is not signifi cant.