Effect on the Proliferation And Apoptosis in Human Breast Cancer Cells by Transfection of Gene HCCR-2

Objective To evaluate the effect and the possible mechanism of human cervical cancer oncogene-2(HCCR-2) transfec tion on cell proliferation and apoptosis of human breast cancer cells.Methods pcDNA3.0-HCCR-2 and pcDNA3.0 were transfected into MCF-7 cells through lipofectamine and positive clones were screened by G418.Western blot were employed to identify protein expression of HCCR-2 in MCF-7 cells.The protein expressions of Bcl-2 and Bax in positive clones were detected by Western blot.The cell proliferation and apoptosis were observed by MTT assay and flow cytomertry.Results Positive clone M-23 with HCCR-2 over-expression was success fully established.Compared with other two groups,the expression of Bcl-2 and the proliferation of M-23 were significantly increased;how ever,the expression of Bax and the apoptosis of M-23 were significantly decreased.Conclusions HCCR-2 transfection can effectively pro mote the proliferation and inhibit the apoptosis of MCF-7 cells by upregulating the expression of Bcl-2 and downregulating the expression of Bax in MCF-7 cells.