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2010•Hubei nongye kexueRequires access

Construction and Identification of Eukaryotic Expression Vector of miR-15a

Ming Duan

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Abstract

A pair of oligodeoxynucleotides was synthesized according to the mature sequence of miR-15a,and cloned into pcDNATM6.2-GW/EmGFP-miR vector to construct pcDNATM6.2-GW/EmGFP-miR-15a.Then the recombinant vector was transfected into lung adenocarcinoma A549 cells,the expression of miR-15a was identified by real-time PCR.The results were helpful to further investigate the biological functions of miR-15a and identify the target genes.

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What this paper is about

A pair of oligodeoxynucleotides was synthesized according to the mature sequence of miR-15a,and cloned into pcDNATM6.2-GW/EmGFP-miR vector to construct pcDNATM6.2-GW/EmGFP-miR-15a.Then the recombinant vector was transfected into lung adenocarcinoma A549 cells,the expression of miR-15a was identified by real-time PCR.The results were helpful to further investigate the biological functions of miR-15a and identify the target genes.

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Available abstract

A pair of oligodeoxynucleotides was synthesized according to the mature sequence of miR-15a,and cloned into pcDNATM6.2-GW/EmGFP-miR vector to construct pcDNATM6.2-GW/EmGFP-miR-15a.Then the recombinant vector was transfected into lung adenocarcinoma A549 cells,the expression of miR-15a was identified by real-time PCR.The results were helpful to further investigate the biological functions of miR-15a and identify the target genes.

Key concepts: Vector (molecular biology), Transfection, Expression vector, Recombinant DNA, microRNA, Biology, Computational biology, Gene

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