The effects of high glucose on chemokine ligand 16(CXCL16) expression and secretion in THP-1 macrophages and intervention of aspirin in the activity

Objective To observe the effects of high glucose on chemokine ligand 16(CXCL16)expression and secretion in THP-1 macrophages and intervention of aspirin in the activity.Methods ① Macrophages was exposed to medium containing 33.6 mmol/L glucose for different time(0,6,12,24,48,72,96h);② Macrophages was exposed to medium containing glouse at different concentration(5.6,11.2,16.8,33.6 mmol/L)for 72 hours.③ Macrophages was exposed to medium containing 3.36 mmol/L glucose and different concentration of asipirin(0,12.5,25,50μg/ml)for 72 hours.RT-PCR and ELISA was used to detect the CXCL16 mRNA expression and protein secretion,respectively.Results 1.With the extension of time,as well as the elevated glucose concentration,CXCL16 expressed and secreted by macrophages increased.2.With the medium of aspirin,concentration of CXCL16 expressed and secreted by macrophage decreased while the concentration of asipirin in the medium increased.Conclusion Aspirin can inhibit the expression and secretion of CXCL16 mediated by high glucose in THP-1 macrophages.