Use of BMP/VEGF in repair of segmental radius defects in rabbits

Objective To investigate the efficacy of combined use of bone morphogenetic protein(BMP) and vascular endothelial growth factor(VEGF) for repairing segmental bone defects. Methods Four kinds of composites: vituline cancellous bone carrier/BMP+PVP/VEGF, vituline cancellous bone carrier/PVP/BMP, vituline cancellous bone carrier/PVP/VEGF and cancellous bone+PVP were made. Next, the four kinds of composites were implanted respectively in the 15-mm-long continuous defects in radius of adult rabbits. The animals were sacrificed at 3,8 and 12 weeks after operation, and the bone repair process was investigated using physical, X-ray film, histological observation and biology mechanics detection. Results Histological and roentgenographic examination showed that vituline cancellous bone carrier/BMP+PVP/VEGF composite repair was more rapid and vigorous, bone density was higher and antibending force was stronger compared with the other 3 groups after 12 weeks. Conclusion The composite of vituline cancellous bone carrier/BMP+PVP/VEGF can efficiently repair bone defects, which can improve not only the vascularization but also the differentiation of primitive cells, so it is the best.