Activation of P38MAPK in the PTZ-induced Rats and Effect of Sodium Valproate

Objective Explore the activation of P38MAPK in the PTZ-induced rats and effect of γ-aminobutyric acid receptor blocker Sodium Valproate.Methods 38 healthy SD rats were randomly divided into 5 groups,control group,epilepsy group and 3 different dose SV group(5mg/kg,10mg/kg,20mg/kg,respectively).Observe seizures situation of rats before and after intraperitoneal injection,according to racine classification standard record,while recording cortical EEG(electrocoti-cogram,ECoG),observe the incubation period of epileptiform discharges and epileptiform discharge duration within one hour.Analysis the expression of P38MAPK in CA1 area,of hippocampus.Expression of P38MAPK,upstream MKK3 and MYC-related protein were detected by Western blot.Results Epilepsy group all have seizure emerged after PTZ,the degree was 5,the degree of attack significantly reduced in the 3 SV groups.Incubation period of ECoG extended,the duration of one hour epileptiform discharges shorted.At the same time,compared with the control group,intensity of protein expression in CA1 area of rats increased in epilepsy group and 3 doses SV groups(P0.05).Compared with the epilepsy group,intensity of protein expression in CA1 area of rats decreased in 3 doses SV groups(P0.05).Conclusion SV has protective effet to PTZ-induced epileptic rats by depression of expression of P38MAPK-associated protein.