Cytotoxicity induced by co-treatment of sodium arsenite and BSO on Chang liver cells

Objective To study the cytotoxicity induced by sodium arsenite(NaAsO2),and co-treatment of NaAsO2 and buthionine sulfoximine(BSO) on Chang liver cells.Methods Cultured Chang liver cells were exposed to NaAsO2 alone or co-exposed to NaAsO2 and BSO for 24hr.Cell morphology and viability were evaluated by inverted phase contrast microscope and MTT assay.Results Cell morphology were changed and cell viability was decreased remarkably with dose-effect relationship under NaAsO2(0-250μmol/L) exposure(P0.01).Co-treatment of NaAsO2(5 and 20 μmol/L) and BSO(1 mmol/L)significantly decreased the cell viability compared with the NaAsO2 exposure groups(P0.01).Conclusion NaAsO2 shows obvious cytotoxicity,while co-treatment of NaAsO2 and BSO aggravates the cytotoxicity on Chang liver cells.