2005•Journal of Nanchang UniversityRequires access
SIMULTANEOUS ULTRA VIOLET SPECTROPHOTOMETRIC DETERMINATION OF SULPHAMETHOXAZOLE AND TRIMETHOPRIM BY MULTIVARIATE CALIBRATION APPROACHES
Yongnian Ni
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Abstract
Sulphonamides generally have overlapping spectra in UV range, and it is difficult to determine them individually. The chemometric multivariate calibration methods, such as partial least squares (PLS) and principal components regression (PCR) were applied to the simultaneous determination of sulphamethoxazole and trimethoprim in mixtures in a B-R buffer solution (pH 9.91) by resolving the overlapping spectra. The linear ranges were 1.0-20.0 and 2.0-24.0 mg/L for sulphamethoxazole and trimethoprim, respectively. The limits of detection were 0.26 and 0.81 mg/L for sulphamethoxazole and trimethoprim, respectively. These methods were successfully applied to the analysis of samples with satisfied results.