Change of IL-8,IL-10 in rats with diabetic nephropathy after irbesartan therapy

Objective To observe the interfering effect of irbesartan on the levels of cytokines correlated with inflammation,interleukin-8 and interleukin-10 in diabetic nephropathy rats,and to investigate the possible drug action mechanism of irbesartan.Methods 36 Wistar rats were randomly divided into three groups:control group of right kidney nephrectomized,diabetic nephropathy group and irbesartan treatment group.There were 12 rats in each group.At the 8th and the 12th week ends,serum and urine of the rats were collected.The levels of interleukin 8,interleukin 10,glucose,creatinine,urea nitrogen in serum and 24-hour urinary albumin in urine were determined.At the same time,the ratio of kidney mass/body mass was determined.Results At the 8th and the 12th week ends,the levels of interleukin 8 in irbesartan treatment group and diabetic nephropathy group were all significantly higher than those in control group,and the level of interleukin 8 in diabetic nephropathy group was significantly higher than that in irbesartan treatment group(26.51±2.34) ng/L vs(16.12±2.11) ng/L,(59.32±1.28) ng/L vs(21.46±2.59) ng/L(P0.01);In irbesartan treatment group and diabetic nephropathy group,the level of interleukin 8 at the 12th week ends was both significantly higher than that at the 8th week end(21.46±2.59) ng/L vs(16.12±2.11) ng/L,(59.32±1.28) ng/L vs(26.51±2.34) ng/L(P0.01);However,the rising format in irbesartan treatment group was less than that in diabetic nephropathy group.At the 8th and the 12th week ends,the levels of interleukin 10 in irbesartan treatment group and diabetic nephropathy group were all significantly lower than that in control group,and the level of interleukin 10 in diabetic nephropathy group was significantly lower than that in irbesartan treatment group(21.27±2.49) ng/L vs(25.58±3.52) ng/L,(18.05±3.55) ng/L vs(33.22±2.97) ng/L(P0.01).In diabetic nephropathy group,the level of interleukin 10 at the 12th week end was significantly lower than that at the 8th week end(18.05±3.55) ng/L vs(21.27±2.49) ng/L(P0.01).But in irbesartan treatment group,the level of interleukin 10 at the 12th week end was significantly higher than that at the 8th week end(33.22±2.97) ng/L vs(25.58±3.52) ng/L(P0.01).At the 8th and the 12th week ends,the levels of interleukin 8 and interleukin 10 in control group showed no significant difference(P0.05).IL-10 was significantly negative correlated with IL-8(r=-0.659,P0.01).IL-8 was significantly positive correlated with glucose,creatinine,urea nitrogen,24-hour urinary albumin and the ratio of kidney mass/body mass(r=0.596,0.764,0.845,0.635,0.830,P0.01),IL-10 was significantly negative correlated with glucose,creatinine,urea nitrogen,24-hour urinary albumin and the ratio of kidney mass/body mass(r=-0.835,-0.739,-0.757,0.745,-0.810,P0.01).Conclusion Irbesartan makes proinflammatory cytokines IL-8 secretion decreased through suppressing the immune inflammatory reaction,meanwhile,it stimulates the secretion of anti-inflammatory cytokines IL-10.Thus,it will contribute to improving renal hypertrophy and decreasing proteinuria of the rats with diabetic nephropathy,react on the protective effect to renal function.