Expression of distal (testis) promoter of EDAG in leukemia cell lines and its regulation during cell differentiation

Objective To explore the regulation mechanism of EDAG expression in leukemia cells. Methods We detected the pattern of EDAG-t mRNA expression via RT-PCR in a panel of leukemia cell lines. Then we further explored the regulation of EDAG-t and EDAG-h expression during the differentiation of K562 and U937 cells. Results EDAG-t (human HEMGN-t) isoform could also be detected in the leukemia cell lines K562 and U937. And both HEMGN-t and HEMGN-h could be down regulated during the differentiation of K562 and U937 cells but the kinetics were different. Conclusion The regulatory mechanisms for HEMGN-t in testis and leukemia cell lines deserve further investigation.