Measurement of Intracellular pH of Gastric Cancer AGS Cell and Effect of Acidic Extracellular Environment on Proliferation and Apoptosis of AGS Cell

Objective:To measure the intracellular pH(pHi) of the gastric cancer AGS cell and investigate the effect of acidic ex-tracellular environment on the proliferation and apoptosis of AGS cell.Methods:The pHi of the AGS cells was detected with a fluores-cent probe, BCECF, through an ion imaging analysis system.The cell viability was determined with MTT method and the proliferation and apoptosis of the cells were studied with flow cytometry.Results:The pHi of AGS cells was obviously alkalified to about 8.60.The viability and the proliferation of the AGS cells were inhibited remarkably and the cells were arrested at S and G2/M phase.No significant changes in cell apoptosis were observed.Conclusions:The gastric AGS cell was significantly alkalified.The number and the viability of AGS cells were decreased by culturing the cells at pH 6.0 condition,which is correlated with the inhibition of cell proliferation but not with cell apoptosis.