Simultaneous Determination of Phenobarbital and Carbamazepine in Serum by HPLC

OBJECTIVE To establish an HPLC method for the determination of Phenobarbital and Carbamazpine in human plasma.METHODS The separation was pertormed on a ODS Hypersil column.The mobile phase was composed of methanol-water(55∶45).The detective wavelength was at 254nm.Phenytoin was used as the internal standard.RESULTS The calibration curves was linear in the range of 2.5～40 μg·mL~(-1) for Phenobarbital(r=0.9996) and 1.25～20 μg·mL~(-1) for Carbamazepine(r=0.9995),respectively.The average recovery of Phenobarbital was 100.69% and that of Carbamazpine was 98.67%.Intra-day RSD and inter-day RSD were less than 3%.CONCLUSION The method is accurate,rapid,and convenient.It can be used for a routine method to determinate the plasma concentration of PB and CBZ.