Effects of TNF-α on Mobilization of bone marrow Mesenchymal Stem Cells on acute lung injury in rat

【Objective】 To evaluate the effects of TNF- α on Mobilization of Mesenchymal Stem Cells(MSCs) on acute lung injury in the peripheral blood(PB) of rats. 【Methods】 24 male and female SD rats were randomly divided into three groups. Control group(CG group, n =6) were intratracheal instillation and subcutaneously injected with sodium chloride. Model of acute lung injury(MG group, n =8) were intratracheal instillation of lipopolysaccharide(LPS 5 mg/kg) and subcutaneously injected with sodium chloride. Interfering group(IG group, n =8) were subcutaneously injected with infliximab after intratracheal instillation of LPS. 5 hour after intratracheal instillation, PB was collected from heart of rats in each group. The density of TNF- α in PB was detect, MSCs were harvested from PB by density gradient centrifugation. The number of the colony forming unit-fibroblasts(CFU- Fs) was counted when peripheral blood mononuclear cells were cultured at 14 d. MSCs were analyzed with multipotent differentiation, which was identified through osteogenic and adipogenic induction. 【Results】 The LPS caused significant increasing in blood serum TNF- α level in model group in comparison with control group(354.0 ± 82.4 vs 38.0 ± 21.6, P 0.05) and decreased in interfering group in comparison with model group(206.5 ± 64.1 vs 354.0 ± 82.4, P 0.05). The results indicated that the number of CFU- F in model group was more than that in control group(3.6 ± 1.47 vs 1.4 ± 2.83, P 0.05), the number of CFU- F in interfering group was decreased compared with model group(1.7 ± 1.72 vs 3.6 ± 1.47, P 0.05). 28 days after osteogenic induction of MSCs, calcium deposition was detected by Alizarin Red, 21 days afteradipogenic differentiation,lipid droplets were detected by oil red. 【Conclusion】 TNF- α has the imobilization effect in MSCs derived from bone marrow in rat model of ALI.