Effect of Survivin Antisense Oligonucleotide on Biological behaviors of Human Malignant Melanoma A375 Cells

Objective To investigate the effects of liposome-mediated survivin antisense oligonucleotide on proliferation and apoptosis of huaman malignant melanoma A375 cells. Methods Survivin phosphorothioate antisense oligodeoxynucleotide (AS-ODN) were synthesized and transfected into the target cells. Proliferation and apoptosis of A375 cells were measured by trypan blue staining、soft agar colony forming assay、electron microscope and flow cytometry. In nude xenograft of cancer,we investigated the tumorigenesis ability of the A375 cell tranfected with survivin antisense oligonucleotide in vivo. Results Transfection of AS-ODN markedly inhibited the proliferation of A375 cells and colony forming compared with M-ODN and S-ODN (P0.05). ASODN treatment resulted accumulation of apoptotic cells and coincident loss of mitotic(G2/M) cells. Malignant proliferative potential of A375 cells was inhibited in nude following transfection with AS-ODN. Conclusion Liposome-mediated Survivin AS-ODN can evidently inhibit cell proliferation and induce apoptosis of A375 cells. Survivin has a significance effect on human malignant melanoma cell division and apoptosis.