ANALYSIS OF IMMUNOPHENOTYPE OF ACUTE LEUKEMIA IN CHILDREN

Objective To analyze the immunophenotype of acute leukemia(AL) in children and explore its clinical significance. Methods The immunophenotype in 46 children with AL wase detected by flow cytometry. Results Of the 46 cases,30 were acute lymphoblastic leukemia(ALL),10 were acute myelogenous leukemia(AML) and six were mixed leukemia.Among the 30 cases of ALL,four were T-lineage ALL(T-ALL)(13.3%) and 26 were B-lineage ALL(B-ALL)(86.7%).The myeloid antigen expression was noted in 10% of ALL with CD33 the most commonly expressed.Among the AML,the lymphoid antigen expression was positive in 40%,with CD7 most commonly expressed.The frequency of the lymphoid antigen expression in AML was higher than that of the myeloid antigen expression in ALL(P0.05).The positive CD34 of the B-ALL was found in 56.5% of the cases,and that of the AML in 40.0%,in which,those of M2 AML cases was 66.6%,higher than those of the cases with other types of AML(28.6%)(P0.05). Conclusion With the immunophenotyping,every acute leukemia case can be better analyzed,especially for those with mixed leukemia and cross-lineage expression leukemia.