Strategies for affinity maturation of antibody using phage display method

Antibodies are favored in the field of diagnosis and targeting medication for their high specificity,therefore,relative industries grow rapidly.As a library construct technology of antibodies,phage display technology can not only be used for the selection of specific antibodies,but also as a technology for affinity maturation of low-affinity antibody.Random recombination of VH and VL is the core of this technology so as to simulate the process of antibody affinity maturation in vivo to some extent.Therefore,there are many advantages of phage display in the improvement of antibody affinity.In this paper,we review the applications of phage display in antibody affinity maturation.