Dynamic observation of cytokine-induced killer cells in peripheral blood of patients infected with chronic hepatitis B

Objective: To oberve dynamically proliferation and cytotoxicity cytokine-induced killer cells(CIKs) in peripheral blood of patients infected with persistent hepatitis B virus.Methods: The peripheral blood was extracted from 10 normal persons(control group) and 20 patients with chronic hepatitis B and peripheral blood mononuclear cells(PBMCs) were isolated routinely.The PBMCs from control group and the patients were co-cultured with IFN-γ,rhIL-2 and anti-CD3mAb,respectively to induce CIK cell generation.The expressions of CD3,CD4,CD8,CD4+CD25,CD3+CD56 and CD28+CD95 on CIK cells were analyzed by flow cytometry on 3,6,12,24 and 30 days after co-culture. Results: The proliferative velocity and killer activity of CIK cells from the patients were lower than those from control group.The proliferative multiple,killer activity and expressions of above surface markers on CIK cells from the patients at various times after co-culture were higher than those before co-culture,and reached peak values on 12 days after co-culture. Conclusion: As compared with control group,the proliferative multiple and killer activity of CIK cells from the patients were low.This may be a reason of resulting in persistent devolpment of HBV infection.