Adaptation of rabies virus aG strain in Vero cells

To provide experimental basis for the preparation of effective,safe and stable rabies vaccine with Vero cells.Subculture fixed rabies virus strain 4aG in Vero cells and explore the condition for virus propagation as well as the method for harvest of virus liquid.After subculture of 4aG strain in Vero cells,an adapted rabies virus strain 4aG-V was obtained,which reached a virulence of 8.50 log LD50/ml and showed that it is of the good antigenicity and immunogenicity.The optimal MOI of 4aG strain in Vero cells is 1∶103.The virus titer increase with the culture time within 12 d and then decrease gradually.Comparing with the titer of virus harvested directly,that of virus liquid harvested by freeze-thaw at low temperature is significantly high.The virus liquid of 4aG-V strain cultured in Vero cells may be harvested for several times,indicating that the strain is suitable for the production of high titer bulk rabies virus.