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2010•Jiangsu Medical JournalRequires access

A study on the mechanism of apoptosis of NB4 cells induced by flavonoids of puerarin

Jian-Min Ji

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Abstract

Objective To explore the effects and the possible molecular mechanism of flavonoids of puerarin(PR) on acute promyelocytic leukemia(APL) cell line NB4 cells in vitro.Methods MTT assay was used to detect the inhibitory effects of cell proliferation.The apoptosis of NB4 cells were detected by flow cytometry marked with AnnexinⅤ/PI.The expressions of JNK,PARP,Bcl-2 and Caspase 3 were detected by Western blot.Results PR could inhibit the proliferation of NB4 cells in time-and dose-dependent manner.The protein expressions of JNK,PARP and Caspase 3 were possitively correlated to the concentration of PR,but negetively correlated to Bcl-2.ConclusionIt is suggested that PR may be a potential anti-leukemia agent that could induce NB4 cells apoptosis and activate JNK signaling pathway.

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What this paper is about

Objective To explore the effects and the possible molecular mechanism of flavonoids of puerarin(PR) on acute promyelocytic leukemia(APL) cell line NB4 cells in vitro.Methods MTT assay was used to detect the inhibitory effects of cell proliferation.The apoptosis of NB4 cells were detected by flow cytometry marked with AnnexinⅤ/PI.The expressions of JNK,PARP,Bcl-2 and Caspase 3 were detected by Western blot.Results PR could inhibit the proliferation of NB4 cells in time-and dose-dependent manner.The protein expressions of JNK,PARP and Caspase 3 were possitively correlated to the concentration of PR,but negetively correlated to Bcl-2.ConclusionIt is suggested that PR may be a potential anti-leukemia agent that could induce NB4 cells apoptosis and activate JNK signaling pathway.

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Available abstract

Objective To explore the effects and the possible molecular mechanism of flavonoids of puerarin(PR) on acute promyelocytic leukemia(APL) cell line NB4 cells in vitro.Methods MTT assay was used to detect the inhibitory effects of cell proliferation.The apoptosis of NB4 cells were detected by flow cytometry marked with AnnexinⅤ/PI.The expressions of JNK,PARP,Bcl-2 and Caspase 3 were detected by Western blot.Results PR could inhibit the proliferation of NB4 cells in time-and dose-dependent manner.The protein expressions of JNK,PARP and Caspase 3 were possitively correlated to the concentration of PR,but negetively correlated to Bcl-2.ConclusionIt is suggested that PR may be a potential anti-leukemia agent that could induce NB4 cells apoptosis and activate JNK signaling pathway.

Key concepts: Annexin, Apoptosis, Puerarin, Western blot, Poly ADP ribose polymerase, Flow cytometry, Chemistry, Cell biology

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