Inhibition of HBsAg Expression in Recombinant CHO Cells by Vector-mediated RNAi

Objective To interfere the expression of HBsAg gene in recombinant CHO cells by vector-mediated RNAi.Methods Design and synthesize two pairs of 64 nt oligo nucleotide fragments according to the sequence of HBV gene and insert downstream to H1 promoter to construct plasmids pHs-9 and pHs-170 for expression of short hairpin RNA(shRNA).Transfect CHO cells integrated with HBsAg gene by the two plasmids and determine the expression level of HBsAg by ELISA.Results Decreased expression levels of HBsAg were determined in the culture supernatant of CHO cells 24-120 h after transfection with plasmids pHs-9 and pHs-170 respectively.Both the inhibitory effect of HBsAg expression reached about 70%.Conclusion The constructed plasmids pHs-9 and pHs-170 successfully expressed shRNA and inhibited the HBsAg expression in recombinant CHO cells.