2000•Europe PMC (PubMed Central)Requires access

The influence of different factors on callus induction of Dioscorea opposita

Mingjun Li, Jianping Xue, Mingxia Chen, Minying Guo, Weihua Qiu, Jiabao Zhang

Abstract

The effects of genotypes, explants, phytohormones, light and dark etc. upon callus induction of Dioscorea opposita were studied. Results showed: (1) Different genotypes of D. opposita could all induce and form calli, but percentages of calli were different. (2) Different explants had obvious diversity on the same medium. The percentages of calli of the same explant were different on the media supplied with different phytohormones concentration. (3) It was crucial for phytohormones to induce callus of D. opposita. Cytokinin and auxin combinations had better effect than single phytohormone, and their concentrations were different, ratio was different, too. (4) The effects of light and dark upon callus induction from different explants were different.

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Key concepts: Callus, Dioscorea, Explant culture, Cytokinin, Auxin, Botany, Horticulture, Biology