2005•Unpublished venueRequires access
Studies of 4500 Umbilical Cord Blood Units Banking
Han Jim-ling, Qian Li, Yang Cong-lin, Min Lü, Han Zhong-chao, Lugui Qiu
Open publisher page 1 citations
Abstract
Objective: To prepare and cryopreserve umbilical cord blood (UCB) units for clinical hematopoietic stem cell transplantation. Methods; HSC of UCB units that met our standards were collected, separated cryopreserved and tested. Results: 4 500 (72. 2%) of 6 232 cord blood units were suitable for preservation. Average net volume, numbers of total nucleated cells, percentage of CD34~+ cells and CFU-C of the UCB units were 90. 7±22. 2 ml, 10. 5±3. 4×10~8, 0. 24±0. 15%, 78.9±44. 14/10~5 NC, respectively. After separation, the cell recovery of nucleated cells, CD34~+ cells and CFU-C were 82. 1±6. 52%, 92. 5±6. 15% and 90±9. 67%, respectively. The cell viability after separating was over 95%. After thawing, the recovery of nucleated cells, CD34~+ cells and CFU-C were 84. 2±10. 1%, 96. 5±21. 8% and 115. 1±23. 3%, respectively. The cell viability was 93. 8±4. 4%. Among the 569 searching cases,532 (93%) cases could found 4/6 of HLA-A, B and DR loci matched UCB units in our bank, including 39 (6. 58%) found 6 loci matched and 237 (41. 65%) found 5 loci matched. Conclusion: These results indicated that we can supply high quality cord blood HSC for clinical transplantation.