Monosaccharide composition analysis and its content determination in polysaccharide extract from Asparagus officinalis

Objective To analyze the monosaccharide composition in the polysaccharide extract from Asparagus officinalis, determine the content of monosaccharide, and provide some references for further research. Methods The monosaccharide composition was determined by HPCE. Phenol-sulfuric method was used for the determination of the content of monofsaccharide. Results The monosaccharide composition in polysaccharides from A. officinalis is Xyl, Fru, Rha, Ara, and Gal, their contents were 3.44%, 7.92%, 10.52%, 17.15%, and 41.85%, respectively. The content of polysaccharide is 79.14%, taking the mixture of monosaccharide compositions as reference substances. Conclusion HPCE can be used to analyze the monosaccharide composition in the polysaccharides with high precision of separation and the method of phenol-sulfuric acid is simple, convenient, and reliable.