Bioremediation of Nitrobenzene-contaminated River Water Using Pseudomonas Putida

Bioremediation of the nitrobenzene polluted river water was studied,using the high effective nitrobenzene degrading pseudomonas putida isolated from sediment of nitrobenzene-contaminated river.The effects of strains presence in the river,addition of nutrients,temperature and nitrobenzene concentration on strain-growth and nitrobenzene degradation were studied.The results indicated that in the control experiment,the nitrobenzene concentration decreased by 5%.At 5℃,pseudomonas putida could mineralize 20 mg/L nitrobenzene in the river water completely in 56 hours,whether sterilized or not.At 25℃,the time of nitrobenzene-degradation was 4 hours shorter when nutrients are added;and 16 hours shorter when it was at 5℃.At 25℃,pseudomonas putida degradated 160 mg/L nitrobenzene completely at a rate of 1.5 mg/L h,utilizing nutrient substance of river without adding of nutrients,and showed the potential for the bioremediation of nitrobenzene-contaminated environment.