The combination detection and significance of HBV-LP and serum hepatitis B virus markers in HBV infected patients

Objective To explore the clinical significance of the combination detection of hepatitis B virus large protein(HBV-LP)and biomakers.Methods For 196 cases of infected serum,HBV-LP was measured by enzymelinked immunosorbent assay,serum HBV-DNA level was quantitatively detected by real-time polymerase chain reaction,and HBV-M was quantitatively detected by time resolved fluoroimmunoassay.Results The positive rates were not significantly different between HBV-LP and HBV-DNA(χ2=0.09,P0.05).The positive rates were significantly different between HBV-LP and HBeAg in the HBV-DNA positive patients(χ2= 68.76,P0.05).HBV-LP absorbance value was remarkablely correlated with the logarithm of HBV-DNA level,the correlate coefficient was 0.97(P0.01,F=104.52).There was no significant difference between the positive rates of HBV-LP and HBV-DNA in the HBeAg negtive patient(χ2=0.74,P0.05).The expressions were not significantly different between HBV-LP and HBV-DNA in various patterns of HBV serological markers either(P0.05).Conclusion Serum HBV-LP is laboratory marker that can accurately reflect HBV-DNA reproduction,and was helpful for complementarity of HBV-M.There is a good correlation between the expression of HBV-LP and the level of HBV-DNA.HBV-LP is valuable for monitoring HBV-DNA replication therapeutic effect and progression of the disease,the combination with the HBV-M can help to the diagnose and treat HBV infected.