2008•The Chinese Journal of Clinical PharmacologyRequires access
Inhibitory effects of Peganum harma/a L.seeds extract and itsβ-carboline alkaloids on the activity of DNA topoisomerase II in vitro
Changhong Wang, Xuemei M. Cheng, Zhongyuan Liu
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Abstract
Objective To study the inhibitory effect of harmine,harma- line,harmane,and total alkaloid of Peganum harmala on the activity of DNA topoisomeraseⅡ.Methods DNA topoisomeraseⅡwas extracted from cultured Q3 hepatocarcinoma cells in vitro.The agarose electropho- resis method was employed to study the inhibitory effect of drugs on the activity of DNA topoisomeraseⅡ,and adriamycin was used as positive control.Results All of the alkaloids of harmine,harmaline,harmane, and total alkaloid of Peganum harmala displayed some inhibitory effect on the activity of DNA topoisomeraseⅡ.Conclusion The inhibitory effect on the activity of DNA topoisomeraseⅡwas one of the mechanisms of the total alkaloid of Peganum harmala against tumour and cytotoxic effect.