Inhibitory effect of colchicine on silica dioxide-induced rat lung fibroblast activation

Aim:To observe the inhibitory effect of colchicine on silica dioxide-induced rat lung fibroblast(LF)activation.Methods:AM was stimulated for 24 hours with silica dioxide suspension,then the supernatant was collected and added colchicine,and the concentration was adjusted to 20,40,and 80 μmol/L.The rat LF was prepared and allocated into 5 groups.The positive control was treated without colchicine.AM supernatant without silica dioxide was used in negative control.The other 3 groups were treated with low,mediated and high-dose colchicine.After 24 h,the mRNA expression levels of the α-SMA,COLⅠ and COL Ⅲ were detected by using RT-PCR,and the protein levels of the COLⅠ,COL Ⅲ and total protein were examined by ELISA.Results:Compared with the positive control,the mRNA expression levels of α-SMA,COLⅠand COL Ⅲ in LF were decreased with the increasing concentration of colchicine,and the high-dose colchicine group changed most significantly(P0.05),so were the content of COLⅠ,COL Ⅲ and total protein in LF culture fluid supernatant(P0.05).Conclusion:Colchicine could decrease the levels of COLⅠand COL Ⅲ and inhibit the effect of LF stimulated by silica dioxide.