Effect of Tanshinone I on HMGB1 Release from Lipopolysaccharide-Stimulated Macrophages

OBJECTIVE To study the effects of tanshinone Ⅰ on extracellular release of high mobility group box protein 1(HMGB1)from lipopolysaccharide-stimulated macrophages and serum HMGB1 level of spesis mice.METHODS The effects of tanshinone Ⅰ(3.6-18.0 μmol·L-1)were observed on HMGB1 release in cultured macrophage-like RAW 264.7 cells and mouse peritoneal macrophages,and on expression of HMGB1 mRNA in cultured macrophage-like RAW 264.7 cells,after these macrophages were stimulated with 100 μg·L-1 LPS.The effects of tanshinone Ⅰ were also investigated on serum HMGB1 level and survival rate of spesis mice which were established using cecal ligation and puncture.RESULTS Tanshinone Ⅰ markedly suppressed the release of HMGB1 from LPS-stimulated macrophage-like RAW 264.7 cells and mouse peritoneal macrophages,and the expression of HMGB1 mRNA in LPS-stimulated macrophage-like RAW 264.7 cells.Tanshinone Ⅰ also significantly decreaseed serum HMGB1 level and elevated survival rate of spesis mice.CONCLUSION Tanshinone Ⅰ suppresses extracellular release of HMGB1,and confers protection against sepsis lethality.