The Application of RAPD Markers in Diversity Detection and Cultivars Identification of Osmanthus fragrans

Based on 28 Primers which are of stable polymorphism selected from 100 random primers, RAPD polymorphism of 34 sweet osmanthus(Osmanthus fragrans)cultivars, 6 individuals from wild population and 5 other species of Osmanthus, and their relationships were detected. Among a total of 231 fragments observed, 209 fragments were polymorphic (90.4%). There were obvious differentiation among different goup in polymorphism, and the order was as follows: Latifolius GroupFragrans Group and Thunbergii GroupAuran-tiacus Group. The results of cluster analysis were consistent with morphological classification by and large. A DNA fingerprinting based on 21 bands amplified with All and M18 was constructed and might be used in sweet osmanthus cultivars identification. There were obvious differentiaion among different cultivars . In addition, it is feasible to identify the sweet osmanthus cultivars and to study their relationships of this cultivars by use of the RAPD technique.