2003•Zhongguo yufang shouyi xuebaoRequires access
Virulent porcine parvovirus rejuvenated by artificial infection swine fetus
Han Xiao-cheng
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Abstract
Porcine Parvovirus virulence was decreased when it was passed 20 generations in 1～5 day porcine kidney primary cell.We challenge adapted cell culture viruses into fetus amniotic cavity by the ventrotomy pregnant sow (pregnant 48 days),after challenged 12days,pregnant sow was slaughtered and viruses were isolated from infectious fetus.We made PPV virulence stronger by such means.