Prediction of New Virulence-Related Genes Adopting Conservative Pathogen-Specific Genes in Bacterial Pathogens

Objective:To predict new virulence-related genes by adopting the method of using the conservative pathogen-specific genes sequence in the bacterial pathogens of similar pathogenic mechanism.Methods:Pathogenic EHEC and EPEC were initially selected with the similar mechanism and their pathogen-specific genes were intercomparisoned in local blasted in the gene fragment databases which was constructed by 115 152 in our laboratory.The obtained pathogen-specific genes were further analyzed with the bacterial pathogens conservative sequence to predict possible virulence-related genes.Results:95 pathogenic bacterial conservative pathogen-specific genes were obtained in 6 strains EHEC,most of which were known pathogenic virulence-related genes and there may be some unknown pathogenic virulence-related genes.10 protein genes were found in the conservative sequences of 9 strips pathogenic EHEC and EPEC,of which there were 9 pathogenic virulence-related genes and 1 possible virulence-related genes.Conclusion:This method was useful to find new virulence-related genes and help investigate the pathogenic mechanism of bacterial pathogens.