Construction of Recombinant Human Interleukin-13 Protein Expression Vector and Its Expression in Escherichia coli

OBJECTIVE To construct recombinant human interleukin 13 protein(rhIL 13) expression vector with its expression in E.coli.METHODS The target cDNA fragment encoding the mature human interleukin 13 (hIL 13) protein was amplified from the total RNA,which were come from activated non adherent human PBMC(peripheral blood mononuclear cells), by nested reverse transcriptase polymerase chain reaction (RT PCR).Then it was cloned into the expression plasmid vector pBV220 containing a thermo inducible promoter P RP L.The constructed expression vector pBV220 containing the hIL 13 cDNA target fragment was named as hIL13 PBV and the engineering bacteria,the E.coli DH5α tranformed with hIL13 PBV was named as hIL13 PBV/DH5α.RESULTS After heat induction for the hIL13 PBV/5α by shifting the culture temperature from 30℃ to 42℃,the recombinant hIL 13(rhIL 13) protein was expressed with a relative molecular mass about 10×10 3 proved by SDS PAGE.CONCLUSIONS Successfully expression of rhIL 13 was propitious to the later researches on the biologic function of IL 13, and providing a substance basis for the clinical application of IL 13 in the future.