Effect of dihydroartemisinin on transplant human HO-8910 ovarian tumor growth in nude mice and Ang-2 expression

Objective To investigate the effect of Dihydroartemisinin(DHA) on HO-8910 human ovarian cancer tumor growth in nude mice and Ang-2 expression.Methods Human HO-8910 ovarian tumor models were established in nude mice,and the mice were divided into the control group,DDP group,DHA high-dose group,DHA low-dose group,DHA with DDP group (n=5).The tumor growth cases,differences in tumor weight and pathological routine examination were observed,and the expression of Ang-2 in tumor tissue were detected by immunohistochemistry.Results Comparing with treatment group,the control group had larger tumor,faster growth,and the differences were statistically significant(P0.05).Comparing with DHA low-dose group,DHA high-dose group,DDP group and combination treatment group were slower growth and weight,and the tumor inhibitory rates were 49.24%,56.06%,75%,respectively.There were significant differences between them(P0.01),and the combined treatment group seems to be better(P0.05).Tumor weight changes between DHA high-dose group and DDP group were no significant differences(P 0.05).Immunohistochemical results suggest that Ang-2 in the treatment group were significantly lower than the control group(P0.05),high-dose group less than the low dose group (P0.05),while the combined treatment group almost no Ang-2 of expression.Conclusion DHA on human ovarian cancer transplanted in nude mice can inhibited tumor growth in a dose-dependent,combining with chemotherapy drugs,the effect should be better.The mechanism may be reduced the expression of Ang-2,which directly or indirectly affects tumor blood vessels building to promote tumor disappeared.