2007•Acta Universitatis Medicinalis NanjingRequires access

Effects of ERK signal pathway on extracellular matrix synthesis of rat mesangial cells treated with PDGF-BB

Weihua Gan

Abstract

Objective:To explore the role of ERK signal pathway in the extracellular matrix(ECM)synthesis of rat mesangial cells treated with PDGF-BB in vitro.Methods:Cultured rat mesangial cells were divided into seven groups:control cells;treated with PDGF-BB(10,25,50 ng/ml,respectively);treated with PDGF-BB(25 ng/ml)and UO126(5,10 μmol/ml,respectively);treated with UO126 10 μmol/ml.The expression of fibronectin(FN),matrix metalloproteinases-2(MMP-2)and plasminogen activator inhibitor-1(PAI-1)mRNAs were detected by RT-PCR.Results:In mesangial cells,PDGF-BB downregulated MMP-2 mRNA expression(P 0.05),upregulated PAI-1and FN mRNAs expression in a dose-dependent way(P 0.05).The changes of FN,MMP-2 and PAI-1 mRNA induced by PDGF-BB can be inhibited by UO126 in a dose-dependent fashion(P 0.05).Conclusion:PDGF-BB can inhibit the degradation of extracellular matrix and stimulate the synthesis of FN in rat mesangial cells through ERK siginal pathway.

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Key concepts: Fibronectin, Platelet-derived growth factor receptor, MAPK/ERK pathway, Extracellular matrix, Mesangial cell, Extracellular, Matrix metalloproteinase, Activator (genetics)