2008•Xinjiang Yike Daxue xuebaoRequires access
The effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth,proliferation and apoptosis in vitro
LI Hai-jun
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Abstract
Objective: To investigate the effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth,proliferation and apoptosis in vitro.Methods: GBC-SD cells were transfected VEGF ASODN and SODN mediated by oligofectamine.The transfected GBC-SD cells of each groups were assessed the change of growth and proliferation activity by MTT,and apoptosis by flow cytometry.Results: The transfected GBC-SD cells growth and proliferation activity tested by MTT in groups of SODN and SODN+oligofectamine had not any difference as compared with control group(P0.05),but as for the cells in groups of ASODN and ASODN+oligofectamine,the growth and proliferation activity were significantly inhibited(P0.05),moreover,the effect of ASODN+oligofectamine was more significant than that of ASODN(P0.05).The results of flow cytometry revealed ASODN+oligofectamine could accelerate transfected GBC-SD cells apoptosis(P0.05).Conclusion: VEGF ASODN could inhibit GBC-SD cells growth,proliferation and accelerate apoptosis.The oligofectamine could strengthen the effect of ASODN.