The influence of TNF-α and IL-1β by different electroacupuncture intensity on acute cerebral ischemia reperfusion in rats

Objective To explore the effects of different intensities of electroacupuncture to the contents of TNF-α and IL-1β on acute cerebral ischemia reperfusion in rats.Methods A total of 44 male SD rats were randomly divided into normal group(n=6),sham operation group(n=8),model group(n=30),model group was randomly divided into model compare group(n=8),weaker electroacupuncture group(n=8),stronger electroacupuncture group(n=8).Established the focal cerebral ischemia reperfusion model(MCAO) by modified suture method in rats,and given Electroacupuncture Baihui,dazhui: sparse-density wave,frequency of 2~15 Hz,adjustment of different current parameters,strong Electroacupuncture Parameters: limb tremor or minor tonic contraction of muscle,and put current controled in 4~7 mA;weak Electroacupuncture parameters: beard fretting or muscle mild beating,and the current controled in 1~3 mA.Then sampling after 24 hours.TNF-α and IL-1β contents of rat serum were measured by enzyme linked immunosorbent assay(ELISA) and did the statistical analysis.Results Compared with the normal group and sham operation group,the contents of TNF-α and IL-1β in model compare group were significantly difference(P0.01);compared with the model compare group,the contents of TNF-α and IL-1β were significantly decrease in the electroacupuncture group(P0.05~0.01);Compared with the weaker electroacupuncture group,the regulation of TNF-α and IL-1β in the stronger electroacupuncture group was significant(P0.05).Conclusion The therapeutic of different intensity electroacupuncture in the regulation of inflammatory cytokines TNF-α and IL-1β have differences in the rats of acute cerebral ischemia reperfusion.The therapeutic of strong stimulation is better than the weak stimulation.