The expression of brain-derived neurotrophic factor in primary cultured astrocytes subjected to hypoxia /reoxygenation

Objective To observe the effect of hypoxia /reoxygenation on primary astrocytes viability and the expression of brain-derived neurotrophic factor. Methods Primary cultured rat cortical astrocytes were randomly divided into two groups: normal control group( N) and hypoxia /reoxygenation group( H /R). At different time points,the changes of cell viability were measured by MTT assay. The levels of BDNF protein were analyzed by western blots,and those in astrocyte-conditioned media were measured by enzyme-linked immunosorbent assay( ELISA). Results Compared with the control group,6 h hypoxia and reoxygenation within 72 h did not affect the astrocyte viability,and did not induce the release of BDNF,but hypoxia /reoxygenation did enhance BDNF expression in astrocytes. Conclusion Hypoxia /reoxygenation enhances the expression of BDNF in primary cultured astrocyte,but can't induce the changes of cell viability and the release of BDNF in vitro.