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2008•Acta Agriculturae Boreali-SinicaRequires access

Zwittermicin A Enhances the Toxicity of Bt Cry1Ac to Spodoptera exigua Hübner

Jianru Shen

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Abstract

Zwittermicin A(ZwA)enhanced the insecticidal activity of Bacillus thuringienesis(Bt) Cry1Ac protein.The mortality was 90% at 48 h after S.exigua larvae were treated with Cry1Ac in the presence of ZwA,which was higher than that without ZwA.All the 5th-instar larvae were dead at 84 h after fed with 5 μg of Cry1Ac plus 25 μg of ZwA(per larvae).Microscopical observation of dissected midgut showed that at 12 h post feeding with Cry1Ac and ZwA no PM were present in the treated larvae,in contrast to the presence of a fully formed PM in the midgut of control larvae.But after 36 h,the midgut displayed normal and PM formed again.

About this research paper

What this paper is about

Zwittermicin A(ZwA)enhanced the insecticidal activity of Bacillus thuringienesis(Bt) Cry1Ac protein.The mortality was 90% at 48 h after S.exigua larvae were treated with Cry1Ac in the presence of ZwA,which was higher than that without ZwA.All the 5th-instar larvae were dead at 84 h after fed with 5 μg of Cry1Ac plus 25 μg of ZwA(per larvae).Microscopical observation of dissected midgut showed that at 12 h post feeding with Cry1Ac and ZwA no PM were present in the treated larvae,in contrast to the presence of a fully formed PM in the midgut of control larvae.But after 36 h,the midgut displayed normal and PM formed again.

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Available abstract

Zwittermicin A(ZwA)enhanced the insecticidal activity of Bacillus thuringienesis(Bt) Cry1Ac protein.The mortality was 90% at 48 h after S.exigua larvae were treated with Cry1Ac in the presence of ZwA,which was higher than that without ZwA.All the 5th-instar larvae were dead at 84 h after fed with 5 μg of Cry1Ac plus 25 μg of ZwA(per larvae).Microscopical observation of dissected midgut showed that at 12 h post feeding with Cry1Ac and ZwA no PM were present in the treated larvae,in contrast to the presence of a fully formed PM in the midgut of control larvae.But after 36 h,the midgut displayed normal and PM formed again.

Key concepts: Cry1Ac, Midgut, Exigua, Bacillus thuringiensis, Spodoptera, Instar, Larva, Biology

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Zwittermicin A Enhances the Toxicity of Bt Cry1Ac to Spodoptera exigua Hübner — Research Paper | ScholarLens