Study on the diabetic model induced by streptozocin in Wistar rats

Objective To explore the optimal experimental conditions of diabetic animal model induced by streptozocin in Wistar rats.Methods Administering streptozocin intraperitoneally to establish high glucose rats animal model.The incidence and mortality of diabetes model were measured.Results On the condition of one-step treatment(40mg/kg;50mg/kg;60mg/kg),the incidence of diabetes in dosage of 50mg/kg was higher.On the condition of two-step treatment(10mg/kg in the first day,administering dosages as same as one-step treatment in the third day),the incidence of diabetes in dosage(10mg/kg at the first time,50mg/kg at the second time) was the highest.Incidence of diabetes in two-step treatment was higher compared with one-step treatment in corresponding dosage group.Sunshine time had effect on both the incidence and mortality of diabetes.When the rats were exposed to sunshine in shorter time,the incidence of diabetes was apparently decsreased and the mortality was increased.Conclusion The animal model of diabetes induced by streptozocin is steady and reliable.On the condition of two-step treatment(10mg/kg in the first day and 50mg/kg in the third day),male wistar rats weighting 190～220g and exposing to enough sunshine more,the incidence of diabetes is the highest.