Simultaneous Determination of Three β-agonists in Mutton by Solid Phase Extraction Coupled with High Performance Liquid Chromatography-Tandem Mass Spectrometry

A soild phase extraction-high performance liquid chromatography-tandem mass spectrophy(HPLC-MS/MS)was developed for determination of three β-agonists such as clenbuterol,ractomine,salbutemol residues in mutton.The samples were extracted by ammonium acetate buffer,and purified by MCX solid-phase extraction column.Using Formic acid-acetonitrile and formic acid-ammonium acetate buffer as mobile phase by gradient elution,the analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization(ESI+)and multiple reaction monitoring(MRM)mode.An external standard was employed for quantitative analysis.The calibration curves were in good linearity between the peak area and the concentration of 2 to 100 ng/mL for three β-agonists with correlation coefficients not less than 0.998 8.The spiked recoveries ranged from 91.3% to 103.8% with relative standard deviations of 2.5% to 5.7%.The proposed method is accurate,sensitive and high-efficient,and is applicable for simultaneous determination of three β-agonists in mutton.