Analysis of genomic structure and introns of CsH1 gene from tea plant

A large-scale screening of cold-induced genes was performed in tea plant(Camellia sinensis(L.)O.Kuntze)by cDNA-amplified fragment length polymorphism(cDNA-AFLP).Based on a stress-induced H1-histone complete cDNA sequence(GenBank accession No.EU716314),the complete DNA sequence was cloned from'Longjing 43',named Camellia sinensis H1-histone gene(CsH1)(JF836005).Gene structure analysis showed that it contained 2 exons and 1 intron,and the splicing principles of its exon and intron were consistent with GT-AG.Two exons were 236 bp and 608 bp,respectively,while the intron was 388 bp.Sequence analysis indicated that the intron had promoter and hormones control components,which may be controlling the specific expression of CsH1 gene.The gene structure and intron of CsH1 gene from tea plant provided sequence information for studies on the expression of cold-induced genes and molecular regulation in cold resistance pathway.