Optimization of propionin production by Response Surface Method

Fermentation process were optimized by using Response Surface Analysis method,the fermentation medium and the cultural conditions can be Examined and evaluated,optimal levels of the variables also can be defined,then the Fermentated activity can beimproved,the fermentation medium for propionin production was optimized by this methed in order to improve the inhibitory activity of propionin.Firstly,three statistically significant parameters including glucose,tween80,pH were identified by Plackett-Burman design.The optimal levels of three variables were defined by Steepest ascent method and Response surface method(RSM): glucose 2.32 g/L,tween80 0.1 g/L,pH 7.0.The inhibitory activity under the optimal fermentation medium can be improved.The diameter of inhibition zone was 24.76 mm,which was as 1.25 times as that under the original fermentation medium.