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Two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii:A comparative study

Yang Chang-mei

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Abstract

Objective To compare the two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.Methods Metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii was detected by modified Hodge test and Etest MBL test.Resistance of Acinetobacter Baumanni to imipenem was assayed with McrioScan WalkAway 96.Results The metallo-β-lactamase was detected in 40 imipenem-resistant Acinetobacter Baumannii strains with the two methods.The metallo-β-lactamase was detected in 36 out of the 40 imipenem-resistant Acinetobacter Baumannii strains by modified Hodge test and in 38 imipenem-resistant Acinetobacter Baumannii strains by Etest MBL test,with a positive detection rate of 90% and 95%,respectively.Conclusion The two detection methods are simple,rapid and accurate for detecting the metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.

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What this paper is about

Objective To compare the two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.Methods Metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii was detected by modified Hodge test and Etest MBL test.Resistance of Acinetobacter Baumanni to imipenem was assayed with McrioScan WalkAway 96.Results The metallo-β-lactamase was detected in 40 imipenem-resistant Acinetobacter Baumannii strains with the two methods.The metallo-β-lactamase was detected in 36 out of the 40 imipenem-resistant Acinetobacter Baumannii strains by modified Hodge test and in 38 imipenem-resistant Acinetobacter Baumannii strains by Etest MBL test,with a positive detection rate of 90% and 95%,respectively.Conclusion The two detection methods are simple,rapid and accurate for detecting the metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.

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Available abstract

Objective To compare the two detection methods of metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.Methods Metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii was detected by modified Hodge test and Etest MBL test.Resistance of Acinetobacter Baumanni to imipenem was assayed with McrioScan WalkAway 96.Results The metallo-β-lactamase was detected in 40 imipenem-resistant Acinetobacter Baumannii strains with the two methods.The metallo-β-lactamase was detected in 36 out of the 40 imipenem-resistant Acinetobacter Baumannii strains by modified Hodge test and in 38 imipenem-resistant Acinetobacter Baumannii strains by Etest MBL test,with a positive detection rate of 90% and 95%,respectively.Conclusion The two detection methods are simple,rapid and accurate for detecting the metallo-β-lactamase produced by imipenem-resistant Acinetobacter Baumannii.

Key concepts: Imipenem, Acinetobacter baumannii, Etest, Microbiology, Acinetobacter, Biology, Medicine, Antibiotics

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