[Relationship between the levels of maternal serum sTNFR II and the mRNA expression of placental TNFR II in preterm labor with chorioamnionitis and polymorphism in -196 site of tumor necrosis factor-beta receptor II].

OBJECTIVE: To investigate the role of Tumor necrosis factor receptor II (TNFR II) in preterm labor with chorioamnionitis and their gene polymorphisms in genetic susceptibility to preterm labor with chorioamnionitis in Chengdu. METHODS: We collected 46 cases maternal serum and partial placental tissues of preterm labor (21 cases of infectious group with chorioamnionitis, 25 cases of noninfectious group without chorioamnionitis), and 50 cases maternal serum and 20 cases placental tissues of term labor in corresponding period. TNFR II mRNA in placental tissue were tested by RT-PCR, maternal serum levels of sTNFR II were measured by ELISA. According to preliminary studies on TNFR II -196 site of the gene type, we analyze the sites of different genotypes in patients with premature placental TNFR II mRNA and maternal blood levels of sTNFR II difference, and with different genotypes chorioamnionitis relevance. RESULTS: In patients with preterm labor, the results of placental TNFR II mRNA and serum sTNFR II were no statistically significant higher in TG (GG) than in TT (P > 0.05). The levels of maternal serum sTNFR II and the mRNA expression of placental TNFR II in preterm labor with chorioamnionitis were significantly higher than those of preterm labor without chorioamnionitis and term labor (P < 0.05). There were no significant difference between term labor and preterm labor without chorioamnionitis (P > 0.05). Close correlation was observed between the different genotypes and the chorioamnionitis (chi2 = 11.088, P<0.05). The odds ratio (OR)for TG + GG genotype was 12.65, 95 CI 2.359-67.848, with more than 12.65 times probability of chorioamnionitis than that of TT genotype group. CONCLUSION: It suggested that TNFR II -196 polymorphism might not play a role by affecting TNFR II production in preterm labor. The site polymorphism is associated with higher serum sTNFR II and placenta TNFR II mRNA expression in patient with chorioamnionitis. It can be a useful marker for early prediction and diagnosis of preterm labor with chorioamnionitis.